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human mirna v19 microarray chip  (Toray Industries)

 
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    Toray Industries human mirna v19 microarray chip
    Cytosolic RNA:DNA hybrids interact with <t>miRNA</t> machinery proteins. A , cytosolic fractions of A549 cells were subjected to immunoprecipitation ( IP ) using RNA:DNA hybrid-specific antibody S9.6. Part of the cytosolic fraction was pretreated with 0.5 units/ml RNase H. Immunoprecipitated proteins were detected by SDS-PAGE and silver staining. The indicated bands were analyzed by mass spectrometry. C , cytosolic; N , nuclear. B and C , A549 cells were treated with DMSO or 10 μ m Ara-C for 15 h and harvested for cell fractionation after fixation. Cytosolic fractions were subjected to immunoprecipitation with RNA:DNA hybrid-specific antibody S9.6. Immunoblot analysis was carried out on immunoprecipitated proteins probed with antibodies specific for DDX17 ( B ) and AGO2 ( C ).
    Human Mirna V19 Microarray Chip, supplied by Toray Industries, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/human mirna v19 microarray chip/product/Toray Industries
    Average 90 stars, based on 1 article reviews
    human mirna v19 microarray chip - by Bioz Stars, 2026-04
    90/100 stars

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    1) Product Images from "RNA Polymerase III Regulates Cytosolic RNA:DNA Hybrids and Intracellular MicroRNA Expression * "

    Article Title: RNA Polymerase III Regulates Cytosolic RNA:DNA Hybrids and Intracellular MicroRNA Expression *

    Journal: The Journal of Biological Chemistry

    doi: 10.1074/jbc.M115.636365

    Cytosolic RNA:DNA hybrids interact with miRNA machinery proteins. A , cytosolic fractions of A549 cells were subjected to immunoprecipitation ( IP ) using RNA:DNA hybrid-specific antibody S9.6. Part of the cytosolic fraction was pretreated with 0.5 units/ml RNase H. Immunoprecipitated proteins were detected by SDS-PAGE and silver staining. The indicated bands were analyzed by mass spectrometry. C , cytosolic; N , nuclear. B and C , A549 cells were treated with DMSO or 10 μ m Ara-C for 15 h and harvested for cell fractionation after fixation. Cytosolic fractions were subjected to immunoprecipitation with RNA:DNA hybrid-specific antibody S9.6. Immunoblot analysis was carried out on immunoprecipitated proteins probed with antibodies specific for DDX17 ( B ) and AGO2 ( C ).
    Figure Legend Snippet: Cytosolic RNA:DNA hybrids interact with miRNA machinery proteins. A , cytosolic fractions of A549 cells were subjected to immunoprecipitation ( IP ) using RNA:DNA hybrid-specific antibody S9.6. Part of the cytosolic fraction was pretreated with 0.5 units/ml RNase H. Immunoprecipitated proteins were detected by SDS-PAGE and silver staining. The indicated bands were analyzed by mass spectrometry. C , cytosolic; N , nuclear. B and C , A549 cells were treated with DMSO or 10 μ m Ara-C for 15 h and harvested for cell fractionation after fixation. Cytosolic fractions were subjected to immunoprecipitation with RNA:DNA hybrid-specific antibody S9.6. Immunoblot analysis was carried out on immunoprecipitated proteins probed with antibodies specific for DDX17 ( B ) and AGO2 ( C ).

    Techniques Used: Immunoprecipitation, SDS Page, Silver Staining, Mass Spectrometry, Cell Fractionation, Western Blot

    Deregulation of miRNAs by Pol III inhibition. A , A549 cells were treated with the Pol III inhibitor ( RP III inh. ) ML-60218 at 10 μ m or DMSO for 24 h. Some cells were further treated with Ara-C for 15 h. A heat map of the global miRNA expression profile from total RNA extracted after treatment is shown. All expression profiles were quantile-normalized, and then -fold values with reference to the control (DMSO) were used for plotting this heat map. 81 probes had at least 3-fold differential expression in any pair of conditions and were preselected for this plot. wrt indicates normalized to. B , scatterplot of miRNA expression profiles between control (DMSO) and ML-6028-treated samples. The decision surface was plotted for at least a 3-fold change to or from the control. Highly up-regulated and down-regulated miRNAs after Pol III inhibitor treatment are identified in the table ( lower rows ). C , A549 cells were treated with 10 μ m ML-60218 or DMSO for 24 h. The expression levels of primary ( Pri-miR ), precursor ( Pre-miR ), and mature miR-4499 from isolated RNA were examined. Student's two-tailed t test was performed. Error bars represent S.E. *, p < 0.05.
    Figure Legend Snippet: Deregulation of miRNAs by Pol III inhibition. A , A549 cells were treated with the Pol III inhibitor ( RP III inh. ) ML-60218 at 10 μ m or DMSO for 24 h. Some cells were further treated with Ara-C for 15 h. A heat map of the global miRNA expression profile from total RNA extracted after treatment is shown. All expression profiles were quantile-normalized, and then -fold values with reference to the control (DMSO) were used for plotting this heat map. 81 probes had at least 3-fold differential expression in any pair of conditions and were preselected for this plot. wrt indicates normalized to. B , scatterplot of miRNA expression profiles between control (DMSO) and ML-6028-treated samples. The decision surface was plotted for at least a 3-fold change to or from the control. Highly up-regulated and down-regulated miRNAs after Pol III inhibitor treatment are identified in the table ( lower rows ). C , A549 cells were treated with 10 μ m ML-60218 or DMSO for 24 h. The expression levels of primary ( Pri-miR ), precursor ( Pre-miR ), and mature miR-4499 from isolated RNA were examined. Student's two-tailed t test was performed. Error bars represent S.E. *, p < 0.05.

    Techniques Used: Inhibition, Expressing, Control, Quantitative Proteomics, Isolation, Two Tailed Test

    RNA transport and mRNA surveillance pathways are potential targets of Pol III-modulated miRNAs. A , a list of predicted miRNAs regulated by Pol III were analyzed for potential gene targets by using the online resource DIANA-mirPath. Yellow boxes indicate genes that are regulated by one miRNA in response to Pol III inhibition. Orange boxes indicate potential target genes regulated by more than one miRNA upon Pol III inhibition. B , mRNA expression levels of three potential genes, each targeted by more than one miRNA. Total RNA was extracted from A549 cells after treatment with the Pol III inhibitor ( RP III inh. ) ML-60218 at 10 μ m or DMSO for 24 h and subjected to real-time PCR analysis. Normalization was done with respect to the HPRT1 housekeeping gene and further compared with DMSO-treated cells. A one-tailed Wilcoxon test was performed. Error bars represent S.E. *, p < 0.05.
    Figure Legend Snippet: RNA transport and mRNA surveillance pathways are potential targets of Pol III-modulated miRNAs. A , a list of predicted miRNAs regulated by Pol III were analyzed for potential gene targets by using the online resource DIANA-mirPath. Yellow boxes indicate genes that are regulated by one miRNA in response to Pol III inhibition. Orange boxes indicate potential target genes regulated by more than one miRNA upon Pol III inhibition. B , mRNA expression levels of three potential genes, each targeted by more than one miRNA. Total RNA was extracted from A549 cells after treatment with the Pol III inhibitor ( RP III inh. ) ML-60218 at 10 μ m or DMSO for 24 h and subjected to real-time PCR analysis. Normalization was done with respect to the HPRT1 housekeeping gene and further compared with DMSO-treated cells. A one-tailed Wilcoxon test was performed. Error bars represent S.E. *, p < 0.05.

    Techniques Used: Inhibition, Expressing, Real-time Polymerase Chain Reaction, One-tailed Test



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    human mirna v19 microarray chip  (Toray Industries)
    90
    Toray Industries human mirna v19 microarray chip
    Cytosolic RNA:DNA hybrids interact with <t>miRNA</t> machinery proteins. A , cytosolic fractions of A549 cells were subjected to immunoprecipitation ( IP ) using RNA:DNA hybrid-specific antibody S9.6. Part of the cytosolic fraction was pretreated with 0.5 units/ml RNase H. Immunoprecipitated proteins were detected by SDS-PAGE and silver staining. The indicated bands were analyzed by mass spectrometry. C , cytosolic; N , nuclear. B and C , A549 cells were treated with DMSO or 10 μ m Ara-C for 15 h and harvested for cell fractionation after fixation. Cytosolic fractions were subjected to immunoprecipitation with RNA:DNA hybrid-specific antibody S9.6. Immunoblot analysis was carried out on immunoprecipitated proteins probed with antibodies specific for DDX17 ( B ) and AGO2 ( C ).
    Human Mirna V19 Microarray Chip, supplied by Toray Industries, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/human mirna v19 microarray chip/product/Toray Industries
    Average 90 stars, based on 1 article reviews
    human mirna v19 microarray chip - by Bioz Stars, 2026-04
    90/100 stars
    		  Buy from Supplier

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    Cytosolic RNA:DNA hybrids interact with miRNA machinery proteins. A , cytosolic fractions of A549 cells were subjected to immunoprecipitation ( IP ) using RNA:DNA hybrid-specific antibody S9.6. Part of the cytosolic fraction was pretreated with 0.5 units/ml RNase H. Immunoprecipitated proteins were detected by SDS-PAGE and silver staining. The indicated bands were analyzed by mass spectrometry. C , cytosolic; N , nuclear. B and C , A549 cells were treated with DMSO or 10 μ m Ara-C for 15 h and harvested for cell fractionation after fixation. Cytosolic fractions were subjected to immunoprecipitation with RNA:DNA hybrid-specific antibody S9.6. Immunoblot analysis was carried out on immunoprecipitated proteins probed with antibodies specific for DDX17 ( B ) and AGO2 ( C ).

    Journal: The Journal of Biological Chemistry

    Article Title: RNA Polymerase III Regulates Cytosolic RNA:DNA Hybrids and Intracellular MicroRNA Expression *

    doi: 10.1074/jbc.M115.636365

    Figure Lengend Snippet: Cytosolic RNA:DNA hybrids interact with miRNA machinery proteins. A , cytosolic fractions of A549 cells were subjected to immunoprecipitation ( IP ) using RNA:DNA hybrid-specific antibody S9.6. Part of the cytosolic fraction was pretreated with 0.5 units/ml RNase H. Immunoprecipitated proteins were detected by SDS-PAGE and silver staining. The indicated bands were analyzed by mass spectrometry. C , cytosolic; N , nuclear. B and C , A549 cells were treated with DMSO or 10 μ m Ara-C for 15 h and harvested for cell fractionation after fixation. Cytosolic fractions were subjected to immunoprecipitation with RNA:DNA hybrid-specific antibody S9.6. Immunoblot analysis was carried out on immunoprecipitated proteins probed with antibodies specific for DDX17 ( B ) and AGO2 ( C ).

    Article Snippet: The labeled RNA was hybridized to a human miRNA V19 microarray chip containing 2019 miRNA probes and analyzed on a ProScanArray microarray scanner (Toray Industries). miRNA profiles were provided as sample-wise median-normalized data by Toray Industries.

    Techniques: Immunoprecipitation, SDS Page, Silver Staining, Mass Spectrometry, Cell Fractionation, Western Blot

    Deregulation of miRNAs by Pol III inhibition. A , A549 cells were treated with the Pol III inhibitor ( RP III inh. ) ML-60218 at 10 μ m or DMSO for 24 h. Some cells were further treated with Ara-C for 15 h. A heat map of the global miRNA expression profile from total RNA extracted after treatment is shown. All expression profiles were quantile-normalized, and then -fold values with reference to the control (DMSO) were used for plotting this heat map. 81 probes had at least 3-fold differential expression in any pair of conditions and were preselected for this plot. wrt indicates normalized to. B , scatterplot of miRNA expression profiles between control (DMSO) and ML-6028-treated samples. The decision surface was plotted for at least a 3-fold change to or from the control. Highly up-regulated and down-regulated miRNAs after Pol III inhibitor treatment are identified in the table ( lower rows ). C , A549 cells were treated with 10 μ m ML-60218 or DMSO for 24 h. The expression levels of primary ( Pri-miR ), precursor ( Pre-miR ), and mature miR-4499 from isolated RNA were examined. Student's two-tailed t test was performed. Error bars represent S.E. *, p < 0.05.

    Journal: The Journal of Biological Chemistry

    Article Title: RNA Polymerase III Regulates Cytosolic RNA:DNA Hybrids and Intracellular MicroRNA Expression *

    doi: 10.1074/jbc.M115.636365

    Figure Lengend Snippet: Deregulation of miRNAs by Pol III inhibition. A , A549 cells were treated with the Pol III inhibitor ( RP III inh. ) ML-60218 at 10 μ m or DMSO for 24 h. Some cells were further treated with Ara-C for 15 h. A heat map of the global miRNA expression profile from total RNA extracted after treatment is shown. All expression profiles were quantile-normalized, and then -fold values with reference to the control (DMSO) were used for plotting this heat map. 81 probes had at least 3-fold differential expression in any pair of conditions and were preselected for this plot. wrt indicates normalized to. B , scatterplot of miRNA expression profiles between control (DMSO) and ML-6028-treated samples. The decision surface was plotted for at least a 3-fold change to or from the control. Highly up-regulated and down-regulated miRNAs after Pol III inhibitor treatment are identified in the table ( lower rows ). C , A549 cells were treated with 10 μ m ML-60218 or DMSO for 24 h. The expression levels of primary ( Pri-miR ), precursor ( Pre-miR ), and mature miR-4499 from isolated RNA were examined. Student's two-tailed t test was performed. Error bars represent S.E. *, p < 0.05.

    Article Snippet: The labeled RNA was hybridized to a human miRNA V19 microarray chip containing 2019 miRNA probes and analyzed on a ProScanArray microarray scanner (Toray Industries). miRNA profiles were provided as sample-wise median-normalized data by Toray Industries.

    Techniques: Inhibition, Expressing, Control, Quantitative Proteomics, Isolation, Two Tailed Test

    RNA transport and mRNA surveillance pathways are potential targets of Pol III-modulated miRNAs. A , a list of predicted miRNAs regulated by Pol III were analyzed for potential gene targets by using the online resource DIANA-mirPath. Yellow boxes indicate genes that are regulated by one miRNA in response to Pol III inhibition. Orange boxes indicate potential target genes regulated by more than one miRNA upon Pol III inhibition. B , mRNA expression levels of three potential genes, each targeted by more than one miRNA. Total RNA was extracted from A549 cells after treatment with the Pol III inhibitor ( RP III inh. ) ML-60218 at 10 μ m or DMSO for 24 h and subjected to real-time PCR analysis. Normalization was done with respect to the HPRT1 housekeeping gene and further compared with DMSO-treated cells. A one-tailed Wilcoxon test was performed. Error bars represent S.E. *, p < 0.05.

    Journal: The Journal of Biological Chemistry

    Article Title: RNA Polymerase III Regulates Cytosolic RNA:DNA Hybrids and Intracellular MicroRNA Expression *

    doi: 10.1074/jbc.M115.636365

    Figure Lengend Snippet: RNA transport and mRNA surveillance pathways are potential targets of Pol III-modulated miRNAs. A , a list of predicted miRNAs regulated by Pol III were analyzed for potential gene targets by using the online resource DIANA-mirPath. Yellow boxes indicate genes that are regulated by one miRNA in response to Pol III inhibition. Orange boxes indicate potential target genes regulated by more than one miRNA upon Pol III inhibition. B , mRNA expression levels of three potential genes, each targeted by more than one miRNA. Total RNA was extracted from A549 cells after treatment with the Pol III inhibitor ( RP III inh. ) ML-60218 at 10 μ m or DMSO for 24 h and subjected to real-time PCR analysis. Normalization was done with respect to the HPRT1 housekeeping gene and further compared with DMSO-treated cells. A one-tailed Wilcoxon test was performed. Error bars represent S.E. *, p < 0.05.

    Article Snippet: The labeled RNA was hybridized to a human miRNA V19 microarray chip containing 2019 miRNA probes and analyzed on a ProScanArray microarray scanner (Toray Industries). miRNA profiles were provided as sample-wise median-normalized data by Toray Industries.

    Techniques: Inhibition, Expressing, Real-time Polymerase Chain Reaction, One-tailed Test